Figure 8
Histological analysis of the control and treated groups by H&E and Mason's trichrome. (a) Neutrophil infiltration, (b) wound healing, (c) granulation tissue formation, (d) epithelialization, (e) collagen level, (f) angiogenesis. The mean difference between the data and the control group of the same group was considered significant at (*p < 0.05 and **p < 0.01). Untreated: Received PBS and treated samples with 4.5 µg ml−1 peptide. Percentage value of mean ± SD of each group. Neutrophil infiltration score 0–3 (none, 0; 0–15% mild, 1; 15–30% moderate, 2; 30–45% severe, 3). Wound reconstruction score was between (1–3). 1 (below 25%) belongs to a poor healing, 2 (25–75%) belongs to medium and (up to 100%) belongs to a perfect healing. Further. The score value of new epithelization is estimated according to the literature. Scales (1–5) 0 = 0% healing, 1 = 1–25%, 2 = 26–50%, 3 = 51–75%, 4 = over 75% but not perfect and 100% assigns to 5. Finally, GT or granulation tissue was defined precisely from to 4 scores. 1 (1–25%) means thin GT, 2 (25–50%) means medium GT, 3 (50–75%) means thick GT, 4 (75–100%) means perfect thick GT similar to intact dermis. Collagen deposition scores: Score: 0, None; 1, scant; 2, moderate; 3, abundant 4. and Angiogenesis scores were described by a graded scale of 0–4 0 mean none and 4 means 100%.
