Figure 4 | Scientific Reports

Figure 4

From: Molecular insights into the dynamic modulation of bacterial ClpP function and oligomerization by peptidomimetic boronate compounds

Figure 4

15% SDS-PAGE after reactions with β-casein monitored for 60 minutes. (a) ixazomib and SeClpP concentrations: 200μM and 10μM, respectively; (b) ixazomib and SeClpP concentrations: 500μM and 10μM, respectively; (c) Comparative experiment with ixazomib (1mM) and ONC206 (10μM), where it is noticeable that the molecular weight (MW) of product fragments vary, according to the ligand used. A possible explanation for this difference is that ixazomib occupies the catalytic sites, affecting the processive degradation of the substrate. ONC206 only binds to the allosteric regions. Degradation products of low molecular weight can be seen in (b) and (c). *t= 0 min corresponds to the time point with no incubation, but until the complete denaturation of ClpP before the SDS-PAGE, β-casein degradation happened in the quick reaction with ONC206. The entire image of each gel is found in Supplementary Fig. S3.

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