Figure 6
ITC measurements with SeClpP and ixazomib, at different molar ratios, (a) in a glycerol-free environment and (b) with 20% w/v glycerol, both at 36°C (the same temperature set for the enzymatic assays). In the presence of glycerol, the ITC data could be fitted, and the following parameters were calculated: N = 9.53 ±0.0993 sites, K = 1.86E5 ±1.99E4 M−1 , \(\Delta {\text{H}}\)= -1.390E4 ±198.0 cal/mol, and \(\Delta {\text{S}}\)= -20.8 cal/mol/deg.
