Figure 7
Spatial control of pit-formation. Opto-RANKc cells were cultured on CaP-coated 48-well plates with blue light exposure every 2 min, for 13 d, without passage. Representative image of pit-formation when (a) the entire well and (b) half of the well was illuminated with blue light. Scale bar: 2 mm. (c and d) Measurement of the pit area in (a) and (b), respectively. The wells were divided into two areas, left and right, and the pit area was measured. Left and right areas in (b) correspond to illuminated and unilluminated areas, respectively. The data show the area of the pits as a percentage of the area of the well halves, represented as mean ± SD from four independent experiments. Data were analyzed using two-tailed unpaired t-test. **, p < 0.01. CaP, calcium phosphate; RANK, receptor activator of nuclear factor-kB.
