Figure 1
Reduced PD-L1 expression after glucose deprivation. (a) HepG2 cells were transfected with either MOCK or pCMV-PD-L1 plasmids. After the specified incubation time, PD-L1, HK2, and β-actin levels were evaluated using western blotting (WB). The full-length blots are in Supplementary Fig. S3. (b) Densitometry intensity ratios for (a) from replicated WB (n = 3). *; p < 0.05, ***; p < 0.001, ****; p < 0.0001. (c) SK-Hep1 cells were treated with the indicated concentration of IFN-γ. After 48 h, PD-L1, HK2, and β-actin levels were evaluated using WB. The full-length blots are shown in Supplementary Fig. S4. (d) Densitometry intensity ratios for (c) from replicated WB (n = 3). *; p < 0.05, **; p < 0.01. (e) HepG2 cells were transfected with pCMV-PD-L1 plasmid. After 48 h, the cells were treated with the indicated concentration of glucose for 8 h. PD-L1, HK2, and β-actin levels were evaluated using WB. The full-length blots are in Supplementary Fig. S5. (f) Densitometry intensity ratios for (e) from replicated WB (n = 3) analyses. *; p < 0.05, **; p < 0.01. (g) SK-Hep1 cells were treated with IFN-γ (15 ng/mL). After 48 h, the cells were treated with the indicated concentration of glucose for 8 h. PD-L1, HK2, and β-actin images were evaluated using WB. The full-length blots are in Supplementary Fig. S6. (h) Densitometry intensity ratios for (g) from replicated WB analyses (n = 3). *; p < 0.05, **; p < 0.01.
