Figure 6 | Scientific Reports

Figure 6

From: Development and validation of cost-effective SYBR Green-based RT-qPCR and its evaluation in a sample pooling strategy for detecting SARS-CoV-2 infection in the Indonesian setting

Figure 6

Validation of the SYBR Green-based RT-qPCR method using a total of 124 clinical samples, comprising 82 positive samples and 42 negative samples. (A) SYBR Green-based RT-qPCR amplification curves for 20 representative positive samples and 10 representative negative samples. (B) Corresponding melt curve dissociation for representative positive and negative samples, unique single peaks with Tm around 82 °C were generated in all positive samples. No nonspecific melt peaks were detected in all negative samples. (C) RT-qPCR amplicon with expected size of 123 bp were specifically amplified for positive samples and no unspecifc amplicon were detected in all negative samples (original gels are presented in Supplementary Fig. 5C).

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