Table 1 Sequence of primers designed for SYBR Green-based RT-qPCR method.
Primer name | Sequences (5'-3') | Primer length (bp) | Product length (bp) | Annealing temp. (°C) | Accession number (bp) |
|---|---|---|---|---|---|
N158 F | TCA CCG CTC TCA CTC AAC AT | 20 | 123 | 59.03 | MZ026854.1 |
N158 R | TTC GTC TGG TAG CTC TTC GG | 20 | 59.19 | ||
N442 F | ACC CGC AAT CCT GCT AAC AA | 20 | 114 | 59.96 | MZ026854.1 |
N442 R | ACG AGA AGA GGC TTG ACT GC | 20 | 60.04 | ||
N1088F | TCC CAC CAA CAG AGC CTA AA | 20 | 107 | 58.56 | MZ026854.1 |
N1088F | TGC AGC AGG AAG AAG AGT CA | 20 | 58.95 | ||
GADPH F | CAA TGA CCC CTT CAT TGA CC | 20 | 155 | 61.68 | NM_002046.7 |
GADPH R | TTG ATT TTG GAG GGA TCT CG | 20 | 60.44 |
