Table 2 Ct value and melting temperatues Tm of clinical samples tested by TaqMan-based and SYBR Green-based RT- qPCR protocols.

1

16.30

12.95

13.60

82.45

2

16.82

13.90

15.18

82.24

3

18.00

15.85

18.65

82.24

4

18.62

16.17

18.18

82.24

5

19.05

16.34

17.82

82.24

6

19.36

16.67

18.86

82.24

7

20.08

17.22

18.03

82.24

8

20.74

18.48

18.22

82.45

9

21.30

18.76

21.77

82.24

10

21.98

19.53

22.87

82.03

11

22.20

20.28

22.35

82.03

12

24.71

21.62

24.68

82.17

13

22.98

21.66

26.10

82.24

14

24.71

20.00

22.08

82.33

15

25.72

22.85

24.07

82.03

16

25.72

22.90

26.05

82.24

17

26.92

22.94

26.59

82.45

18

29.96

27.66

29.07

82.24

19

32.41

29.73

31.11

82.24

20

32.53

30.37

33.27

82.24

21

nd

nd

nd

–

22

nd

nd

nd

–

23

nd

nd

nd

–

24

nd

nd

nd

–

25

nd

nd

nd

–

26

nd

nd

nd

–

27

nd

nd

nd

–

28

nd

nd

nd

–

29

nd

nd

nd

–

30

nd

nd

nd

–

31

32.20

31.72

34.88

81.95

32

31.28

28.86

29.08

81.95

33

27.79

27.45

32.34

82.16

34

30.04

28.08

32.05

82.38

35

31.00

29.83

33.59

82.59

36

30.04

31.57

34.07

82.16

37

nd

nd

nd

–

38

32.54

30.87

35.54

82.16

39

nd

nd

nd

–

40

nd

nd

nd

–

41

nd

nd

nd

–

42

32.39

32.82

34.71

81.95

43

30.47

32.49

34.47

81.09

44

nd

nd

nd

–

45

nd

nd

nd

–

46

nd

nd

nd

–

47

20.16

20.43

23.44

82.38

48

nd

nd

nd

–

49

27.75

26.79

29.77

81.30

50

32.31

31.06

34.56

82.38

51

31.51

31.32

35.86

82.16

52

33.46

29.73

30.21

82.16

53

nd

nd

nd

–

54

30.07

29.69

31.21

82.16

55

30.54

30.12

34.16

81.95

56

nd

nd

nd

–

57

nd

nd

nd

–

58

28.09

29.46

33.54

81.95

59

20.63

20,89

22.37

82.16

60

30.33

29.33

29.93

82.16

61

20.60

21.15

23.84

82.16

62

31.35

30.07

33.61

81.73

63

33.26

32.22

34.29

81.52

64

31.16

29.29

34.13

81.73

65

27.08

27.19

30.47

81.73

66

24.43

25.32

29.04

81.73

67

28.58

27.41

30.14

81.73

68

31.52

30.98

35.82

81.95

69

33.80

33.47

33.38

81.95

70

32.31

31.16

34.42

81.73

71

nd

nd

nd

–

72

nd

nd

nd

–

73

17.6

18.06

23.13

82.31

74

nd

nd

nd

–

75

20.29

21.36

25.88

82.52

76

nd

nd

nd

–

77

25.5

27.6

31.00

82.09

78

17.8

19.8

21.91

82.52

79

29.70

31.20

32.81

82.09

80

19.20

20.05

23.30

82.09

81

27.70

30.11

31.81

82.09

82

30.12

29.55

32.04

82.09

83

nd

nd

nd

–

84

nd

nd

nd

–

85

nd

nd

nd

–

86

nd

nd

nd

–

87

nd

31.22

34.22

82.52

88

19.08

20.08

25.40

82.09

89

17.88

19.30

23.17

82.30

90

15.90

17.00

21.50

82.09

91

nd

nd

nd

–

92

27.11

28.40

31.28

82.74

93

nd

nd

nd

–

94

nd

nd

nd

–

95

19.77

21.80

22.69

82.52

96

nd

nd

nd

–

97

–

31.59

35.86

82.16

98

nd

nd

37.69

78.72*

99

nd

nd

nd

–

100

nd

nd

nd

–

101

–

34.46

35.31

81.95

102

nd

nd

nd

–

103

nd

nd

nd

–

104

nd

nd

37.81

77.21*

105

31.08

28.50

30.22

81.95

106

nd

nd

nd

–

107

nd

nd

nd

–

108

27.75

26.62

32.53

82.16

109

nd

nd

nd

–

110

27.47

27.36

33.62

82.38

111

19.06

19.07

24.36

82.38

112

16.06

17.47

22.56

82.38

113

20.13

20.62

26.84

82.38

114

15.12

16.02

21.80

82.38

115

27.17

27.55

33.04

81.95

116

18.26

18.69

23.40

82.16

117

23.88

24.76

28.21

82.16

118

27.48

29.74

33.76

81.73

119

16.69

17.31

22.16

82.38

120

28.82

30.47

33.14

82.16

121

27.22

29.54

33.25

82.16

122

29.99

31.86

32.12

82.16

123

30.99

29.34

32.40

82.16

124

21.20

21.25

23.74

82.59