Table 1 List of selected candidate reference genes and the gene of interest (GOI) analysed in the HMC3 cell line.

From: Selection of suitable reference genes for gene expression studies in HMC3 cell line by quantitative real-time RT-PCR

Gene symbol

GenBank accession number

Gene function (GO terms)

Primer pair (5´ → 3´)

ACTB

NM_001101.5

Structural constituent of cytoskeleton

AGACCTGTACGCCAACACAGT

AGTACTTGCGCTCAGGAGGA

B2M

NM_004048.4

Peptide antigen assembly with MHC class II protein complex

GCGGGCATTCCTGAAGCTGACAGCA

CTTTCCATTCTCTGCTGGATGACG

GAPDH

NM_002046.7

Glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity

CAACGACCACTTTGTCAA

CTGTGAGGAGGGGAGATTCA

IL-1β

NM_000576.3

Cytokine with pro-inflammatory activity

TGATGGCTTATTACAGTGGCAATG

GTAGTGGTGGTCGGAGATTCG

HMBS

NM_000190.4

Hydroxymethylbilane synthase activity

CGGCTGCAACGGCGGAAGA

GTCCCCTGTGGTGGACATAGC

HPRT1

NM_000194.3

Hypoxanthine phosphoribosyltransferase activity

CAGCCCTGGCGTCGTGATTAGT

ATCTCGAGCAAGACGTTCAG

RPL13

NM_000977

Structural constituent of ribosome

TGAAGGAGTACCGCTCCAAAC

GGAGACTAGCGAAGGCTTTGA

RPS18

NM_022551

Structural constituent of ribosome

CAGAAGGATGTAAAGGATGG

TATTTCTTCTTGGACACACC

TBP

NM_003194.5

RNA polymerase II general transcription initiation factor activity

GTGATCTTTGCAGTGACCCAG

CTGGAACTCGTCTCACTATTCA

TMEM199

NM_152464.3

Lysosomal protein catabolic process

CCTCCACGGAACCCAGAACTA

TGAAGATGGTGATGACCAGAGC

YWHAZ

NM_001135699

Phosphoserine residue binding

TCCCGTTTCCGAGCCATAAA

TGACCTACGGGCTCCTACAA

Back to article page