Table 2 Stability values determined by ∆CT method (Mean SD values), BestKeeper (SD values), Normfinder (stability values), geNorm (M values) and RefFinder (Geomean), for ten candidate reference genes in the HMC3 microglia cell line across all the experimental samples.

From: Selection of suitable reference genes for gene expression studies in HMC3 cell line by quantitative real-time RT-PCR

Gene

∆CT (SD)

BestKeeper (SD)

Normfinder (stability value)

geNorm (M value)

RefFinder (Geomean)

ACTB

1.29

0.96

0.378

0.6639

9.74

B2M

0.93

0.34

0.140

0.7068

3.87

GAPDH

1.51

0.98

0.315

1.2049

8.74

HMBS

1.21

1.09

0.532

0.8739

8.46

HPRT1

0.81

0.24

0.069

0.5613

1.68

RPL13

0.99

0.44

0.163

0.6326

4.36

RPS18

0.94

0.26

0.230

0.4765

1.86

TBP

0.98

0.49

0.229

0.5032

2.00

TMEM199

1.03

0.52

0.298

0.5528

5.96

YWHAZ

0.96

0.65

0.199

0.5262

6.74

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