Table 4 Expression stability ranking of ten RGs in HMC3 cell line:independent analyses and comprehensive ranking using geometric mean of ranks.
Ranking | ∆CT (SD) | BestKeeper (SD) | Normfinder (Stability value) | geNorm (M value) | RefFinder (Geomean) | Final ranking (Geomean) |
|---|---|---|---|---|---|---|
1 | HPRT1 | HPRT1 | HPRT1 | RPS18 | HPRT1 | HPRT1 |
2 | B2M | RPS18 | B2M | TBP | RPS18 | RPS18 |
3 | RSP18 | B2M | RPL13 | YWHAZ | TBP | B2M |
4 | YWHAZ | RPL13 | YWHAZ | TMEM199 | B2M | TBP |
5 | TBP | TBP | TBP | HPRT1 | RPL13 | RPL13 |
6 | RPL13 | TMEM199 | RPS18 | RPL13 | TMEM199 | YWHAZ |
7 | TMEM199 | YWHAZ | TMEM199 | ACTB | YWHAZ | TMEM199 |
8 | HMBS | ACTB | GAPDH | B2M | HMBS | ACTB |
9 | ACTB | GAPDH | ACTB | HMBS | GAPDH | HMBS |
10 | GAPDH | HMBS | HMBS | GAPDH | ACTB | GAPDH |
