Figure 6

Profiles of growth (A) and immune (B,C) related genes expression of olive flounder as measured by RT-qPCR. The gene expression level of growth hormone (GH) was investigated in the brain, and the gene expression of growth hormone receptor (GHR) was investigated in the kidney (GHT-K) and intestine (GHT-I). Immune-related gene expression was investigated in both kidney (B) and intestine (C). Gene expression was quantified relative to β-actin transcription. Data represent the mean ± standard deviation of 3 replicates (3 fish/replicate); ns means no significant difference (P > 0.05) and star means significant difference (P < 0.05). FM70 basal diet, FM35 low-fishmeal diet, HALP low-fishmeal diet supplemented with host-associated low-temperature probiotics, IL interleukin, TNF tumor necrosis factor.