Figure 3 | Scientific Reports

Figure 3

From: A cost-effective RNA extraction and RT-qPCR approach to detect California serogroup viruses from pooled mosquito samples

Figure 3

Reproducibility of California serogroup virus screening results via TRIzol, QIAGEN, and MACHEREY–NAGEL RNA extraction and subsequent RT-qPCR. Panel A depicts the percentage of samples that have been recaptured as positive for California serogroup viruses (CSGv) for each method. The checkered pattern indicates samples that were tested positive for CSGv in only one of the two TRIzol replicates and were not detected in either of the QIAGEN and MN replicates. Panel B shows that RNA yield (logged) and mean Cq values from the RT-qPCR were not correlated. Note that samples tested (N = 40) were initially screened positive by the National Microbiology Laboratory in Winnipeg, Manitoba, Canada and Carleton University, Ottawa, Ontario, Canada.

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