Table 1 Summary of internal control performance across mosquito species.
Speciesa | Mean Cq value (18S internal control)b | ||
|---|---|---|---|
TRIzol | QIAGEN RNeasy | MN Nucleospin | |
Aedes cinereus | 28.7 | 29.2 | 26.7 |
Aedes vexans | 26.6 | 27.8 | 27.9 |
Anopheles punctipennis | 22.9 | 25.7 | 20.6 |
Anopheles quadrimaculatus | 25 | 35.5 | 19.3 |
Anopheles walkeri | 24.5 | 24.1 | 20.3 |
Coquillettidia perturbans | 25 | 24.2 | 23.4 |
Culex pipiens-restuans | 29.1 | 26.7 | 25.8 |
Culex restuans | 26 | 29.8 | 22.5 |
Culex salinarius | 26 | 29.3 | 29.2 |
Culiseta morsitans | 30 | 29 | 26.6 |
Ochlerotatus abserratus-punctor | 25.1 | 26.1 | 28.1 |
Ochlerotatus canadensis | 27.7 | 27.6 | 24.6 |
Ochlerotatus communis | 20 | 28.9 | 19.4 |
Ochlerotatus dorsalis | 31.7 | 22.6 | 22.3 |
Ochlerotatus implicatus | 28.9 | 28.1 | 25.8 |
Ochlerotatus intrudens | 29.3 | 31 | 25.7 |
Ochlerotatus provocans | 27.2 | 29.8 | 27.8 |
Ochlerotatus punctor | 27.1 | 33 | 26 |
Ochlerotatus sticticus | 31.2 | 34.8 | 26.6 |
Ochlerotatus stimulans | 22.3 | 22.2 | 24.9 |
Ochlerotatus triseriatus | 26.6 | 25.1 | 23.7 |
Ochlerotatus triseriatus-hendersoni | 22.7 | 23.1 | 21.5 |
Ochlerotatus trivittatus | 36.3 | 26.2 | 30.6 |
Psorophora ferox | 35.5 | 28.2 | 29.3 |
Uranotaenia sapphirina | 34.4 | 28.5 | 20.6 |
