Figure 2
Ductular reaction and fibrosis are increased in cholestatic mice. (A) Representative images of FPPE liver tissue sections from WT, Trailfl/fl, and TrailΔmye mice were immunostained for the cholangiocyte marker, CK19. Scale bar = 50 µm. Right panel displays the quantified data of digital image analysis of immunostained sections. (B) Representative images of FPPE mouse liver tissue sections immunostained for the myofibroblast marker, desmin. Scale bar = 150 µm. Digital image quantification is presented in the right panel. (C) Representative images of FPPE mouse liver sections stained for fibrosis with Sirius Red. Fast green was used as counterstain. Scale bar = 100 µm. Collagen was quantified using Sirius Red under plane-polarized light and is presented in the right panel. (D) Representative images of frozen mouse liver sections immunostained for the fibrillar collagen, COL1A1. The right panel shows the digital image quantification. (*p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001).
