Figure 8

Light micrograph demonstrating H and E stained colorectal sections, (A) control group showing normal colorectal glandular tissue with pseudostratified ciliated columnar epithelium enclosing lumen (L), no cell atypia, and stroma (S). (B–D) positive control group of colorectal tissue with adenocarcinoma showing loss of normal glandular architecture, poor-differentiated adenocarcinoma (arrows), hyperplasia of glandular epithelium (*) with mucinous areas, lumen (L), and abundant tumor-infiltrating lymphocytes (IF). (E) illustrating colorectal tissue after applying sericin as a prophylactic showing tubular glandular form with epithelial hyperplasia (*), lumen (L), and stroma (S). (F) demonstrating colorectal tissue after applying sericin as a treatment showing poor-differentiated glandular form (arrows), lumen (L), and infiltrating lymphocytes (IF). (G) illustrating colorectal tissue after applying propolis as a prophylactic showing epithelial hyperplasia (*) with mucinous areas, dysplasia of the normal glandular form (arrows) with damaged epithelium, and stroma (S). (H) demonstrating colorectal tissue after applying propolis as a treatment showing near to normal appearance of glandular epithelium with lymphatic invasion (IF), lumen (L) and stroma (S). (I) colorectal tissue after applying nano sericin/propolis as a prophylactic showing numerous tumor-infiltrating lymphocytes (IF) with mucinous areas appearing in the neoplastic epithelium (arrows), damaged epithelium (arrowhead), lumen (L) and stroma. (J) colorectal tissue after applying nano sericin/propolis as a treatment illustrating marked nuclear atypia (arrows) and a high nucleus-to-cytoplasm ratio, nuclei were moderately to highly pleomorphic, and lumen (L). (K) demonstrating colorectal tissue after applying fluorouracil as a treatment showing necrotic glandular epithelium, lymphocytic infiltration (IF), and stroma (S). (L) colorectal tissue after applying nano sericin/propolis/fluorouracil as a treatment illustrating marked multiple lumens (arrows) with mucinous areas, and lymphocytic infiltration (IF).