Table 1 Primers’ sequences of target genes in vitro and in vivo.
Study | Tested gene | Primer sequence |
|---|---|---|
In vitro | P53 | F: CCTCAGCATCTTATCCGAGTGG |
R: TGGATGGTGGTACAGTCAGAGC | ||
PI3K | F: GGTTGTCTGTCAATCGGTGACTGT | |
R: GAACTGCAGTGCACCTTTCAAGC | ||
BCL-2 | F: ATCGCCCTGTGGATGACTGAGT | |
R: GCCAGGAGAAATCAAACAGAGGC | ||
mTOR | F: GCTTGATTTGGTTCCCAGGACAGT | |
R: GCTTGATTTGGTTCCCAGGACAGT | ||
AKT | F: TTCTGCAGCTATGCGCAATGTG | |
R: TGGCCAGCATACCATAGTGAGGTT | ||
PGC-1α | F: CCAAAGGATGCGCTCTCGTTCA | |
R: CGGTGTCTGTAGTGGCTTGACT | ||
VEGF | F: TTGCCTTGCTGCTCTACCTCCA | |
R: GATGGCAGTAGCTGCGCTGATA | ||
PTEN | F: GGTTGCCACAAAGTGCCTCGTTTA | |
R: CAGGTAGAAGGCAACTCTGCCAAA | ||
β-actin | F: CACCATTGGCAATGAGCGGTTC | |
R: AGGTCTTTGCGGATGTCCACGT | ||
In vivo | Caspase 3 | F: GTGGAACTGACGATGATATGGC |
R: CGCAAAGTGACTGGATGAACC | ||
Caspase 9 | F: AGTTCCCGGGTGCTGTCTAT | |
R: GCCATGGTCTTTCTGCTCAC | ||
Bax | F: CGGCGAATTGGAGATGAACTGG | |
R: CTAGCAAAGTAGAAGAGGGCAACC | ||
BCL-2 | F: TGTGGATGACTGACTACCTGAACC | |
R: CAGCCAGGAGAAATCAAACAGAGG | ||
β-actin | F: AAGATCCTGACCGAGCGTGG | |
R: CAGCACTGTGTTGGCATAGAGG |
