Figure 4

Differential communication analysis between CSC subgroups and TAMs. The t-SNE plot shows the three OSC subclusters and three TAM subclusters. (A,B) Dot plots showing the ligand–receptor interactions across the three OSC subclusters and three TAM subclusters. The size of the dots indicates the proportion of cells expressing the marker. The colored dots represent the mean expression levels of the markers. (D) Heatmap showing the outgoing signaling patterns and incoming signaling patterns of the OSC–TAM communication (https://cran.r-project.org/web/packages/pheatmap/index.html). (C,E) Ligand–receptor interaction plot for the four OS subclusters. The thickness of the line represents the relative expression levels from high to low. (F) Differential communication pattern analysis.