Figure 2 | Scientific Reports

Figure 2

From: Downregulation of Roundabout guidance receptor 2 suppresses hepatocellular carcinoma progression by interacting with Y-box binding protein 1

Figure 2

Effects of knocking down Robo2 on EMT and proliferation of hepatoma cells. (A–C) The expression of E-Cadherin and α-SMA in HepG2 and Huh7 cells was detected by Western blot. (D, E) The cell cycle changes after knocking down Robo2 were detected by flow cytometry. (F–H) The protein expression level of PCNA, a proliferation marker, was detected by Western blot. (I, J) The scratch healing experiment was carried out in Robo2-knock-down hepatoma cells. E-Cadherin, α-SMA and PCNA protein levels (normalized to GAPDH) were measured by scanning densitometry. *P < 0.05 vs indicated groups. The samples derive from the same experiment and that blots were processed in parallel. Original blots are presented in Supplementary Fig. 3.

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