Figure 6 | Scientific Reports

Figure 6

From: Downregulation of Roundabout guidance receptor 2 suppresses hepatocellular carcinoma progression by interacting with Y-box binding protein 1

Figure 6

Robo2 regulates cell apoptosis by interacting with YB-1. (A, B) Apoptosis was tested by TUNEL staining in Lv-shNC + Lv-NC, Lv-shNC + Lv-YB-1, Lv-shRobo2 + Lv-NC and Lv-shRobo2 + Lv-YB-1 groups of HepG2 and Huh7 cells. (C, D) Annexin V-APC and PI double staining was utilized to detect the apoptosis. (E–H) Western blot was applied to test the expression of cleaved-PARP in the above four groups of HCC cells. Cleaved-PARP protein levels (normalized to GAPDH) were measured by scanning densitometry. *P < 0.05, **P < 0.01 vs indicated groups. The samples derive from the same experiment and that blots were processed in parallel. Original blots are presented in Supplementary Fig. 6.

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