Figure 4
From: HBV promotes its replication by up-regulating RAD51C gene expression
RAD51C proteins in the nucleus interacted with HBX proteins. (A) HEK293T cells were co-transfected with pFlag-tag, pFlag-HBE, pFlag-HBC, pFlag-HBX, pFlag-HBP, pFlag-HBs(L), pFlag-HBs(S), and HA-RAD51C, then cultured for 48 h, collected, and prepared as a whole-cell lysate for Co-IP. Co-IP reactants were detected by western blot. (B) HA-RAD51C and Flag-HBX plasmids were co-transfected into Huh7 cells; nuclei were stained with green fluorescent-labeled specific HA secondary antibodies, red fluorescent-labeled specific Flag secondary antibodies, and DAPI to observe localization by confocal fluorescence microscopy. Scale bar = 20 µm.
