Figure 3 | Scientific Reports

Figure 3

From: Alternative conformations of a group 4 Late Embryogenesis Abundant protein associated to its in vitro protective activity

Figure 3

AtLEA4-5 mutants where the most LEA4 protein conserved residues were changed. (a) Schematic localization of the point mutations in each AtLEA4-5 mutant. (b) CD spectra of mutant proteins in 80% glycerol solution. Inset shows the corresponding differential spectra. [Φ] = Molar ellipticity. (c) Dichroweb analysis showing the fractions of different secondary structures in wild-type and mutant proteins. (d) Protecting activity of wild-type and mutant proteins in freeze–thaw assays using LDH as reporter enzyme. These data was obtained from three independent experiments with three technical replicates, using 10:1 LEA:LDH molar ratio. Error bars represent the standard deviations between samples. ns non statistically difference as compared to LEA4-5.

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