Figure 8 | Scientific Reports

Figure 8

From: Identification of potential inhibitor against Leishmania donovani mitochondrial DNA primase through in-silico and in vitro drug repurposing approaches

Figure 8

Optimization of parameters for the primase-pyrophosphatase assay. (A) Activity of primase at varied DNA concentrations. (B) Primase activity assessed in presence of divalent metals salts at 2 mM concentration. (C) Primase activity with buffers at pH range 6–10. (D) Time course representation of the priming reaction over period of 0-70 min. (E) Rate PPi released by primase as a function of NTP concentrations. The experiments were conducted over a duration of 30 min.

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