Figure 5

Actin cytoskeleton staining of DAM-exposed SMCs. F-actin and nuclei staining 5 and 50min after addition of 1mM DAM. (A) Untreated control, (B) 1mM DAM, (C) 10µM ML161 treatment, (D) 20µM Y-27632 and (E) 10µM AX treatment prior to DAM addition. (F) Quantification of the fraction of condensed SMCs. Single asterisks indicate comparison to untreated control. Brackets indicate comparisons between experimental groups. n=3. *p<0.05, **p<0.01, ***p<0.001.