Figure 3 | Scientific Reports

Figure 3

From: Cross-regulation of Aps-promoters in Lacticaseibacillus paracasei by the PsdR response regulator in response to lantibiotics

Figure 3

Gene induction in the presence of nisin. The transcriptional response to nisin of the bce-like resistance genes, dltA and mprF in ΔpsdRΔapsR was analyzed. Cell cultures at mid-exponential growth phase were treated with 22.5 ng ml−1 nisin for 10 min and qRT-PCR was performed to determine the expression level of the genes indicated on the X-axis. Relative transcript levels of the genes 10 min after nisin addition were compared to the levels of the same strain in the absence of nisin. The means and standard deviations (error bars) were obtained from biological triplicates. For comparison, the gene expression profiles in response to the same concentration of nisin of the strains ΔpsdR and ΔapsR previously reported17 are also presented.

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