Figure 1
From: Innovative high fat diet establishes a novel zebrafish model for the study of visceral obesity
Overview of zebrafish feeding trial and dietary groups. This figure illustrates the experimental setup of the zebrafish feeding trial, assessing the nutritional impact of various diets. All subjects were provided with five daily feedings of their specific diets, each supplemented with a daily serving of Artemia salina. The study included: NOD Control Group: Maintained on a GEMMA standard diet (SKRETTING, Stavanger, Norway) with 14% fat. HFD40 Juvenile (HFD40 juv.) and HFD60 Juvenile (HFD60 juv.) Groups: Started high-fat diets from the second week post-fertilization and fed diets with 40% and 60% Beef Fat Powder (GRAU GmbH, Isselburg, Germany), each blended with 10% magnesium aluminometasilicate. HFD40 Adult (HFD40 ad.) and HFD60 Adult (HFD60 ad.) Groups: Began high-fat diets from the 12th week post-fertilization, fed identical compositions as their juvenile counterparts, with corresponding 40% and 60% Beef Fat Powder concentrations. Each group's dietary regimen reflects a distinct blend tailored to evaluate the effects of varying fat concentrations on zebrafish development and health. Figure generated using BioRender.com.
