Figure 6

Histological evaluation of zebrafish liver tissue subjected to various dietary regimens. This figure illustrates the histopathological differences in the liver tissue among the zebrafish groups subjected to different dietary conditions, highlighting the impact of the high-fat diet on liver health. Liver sections were stained with Goldner's trichrome stain to examine cellular morphology. Control group (NOD): Zebrafish fed a standard commercial diet (GEMMA) exhibited typical liver architecture with varying degrees of minimal vacuolation. HFD40 juvenile group (HFD40 juv.) Zebrafish starting a high-fat diet from the second week post-fertilization were fed a diet blended with 40% Beef Fat Powder and 10% magnesium aluminometasilicate, and showed mild steatosis with mild vacuolation. HFD60 juvenile group (HFD60 juv.) Juvenile zebrafish, starting a high-fat diet from the second week post-fertilization, were fed a diet blended with 60% Beef Fat Powder and 10% magnesium aluminometasilicate, and displayed mild steatosis with moderate vacuolation. HFD40 adult group (HFD40 ad.) Adult zebrafish starting a high-fat diet from the 12th week post-fertilization were fed a diet blended with 40% Beef Fat Powder and 10% magnesium aluminometasilicate, demonstrating noticeable moderate steatosis but without large vacuolation. HFD60 adult group (HFD60 ad.) Adult zebrafish, starting a high-fat diet from the 12th week post-fertilization, were fed a diet blended with 60% Beef Fat Powder and 10% magnesium aluminometasilicate, showing mild to moderate steatosis with moderate vacuolation. All groups supplemented with Artemia salina. Key histological features, such as the 'muralium duplex' arrangement—yellow arrowheads, hepatocyte vacuolation—white arrows, and steatosis—black arrows, are indicated. Scale bars represent 100 µm. These images provide a comparative insight into the varying degrees of liver steatosis induced by different high-fat diets.