Table 2 Statistics of raw data, trimmed data, original assembly, and transcriptome assembly of telencephalon transcriptome from C. macropomum.
Raw data | |
Number of raw reads | 107,450,263 |
Total Bases (bp) | 83,1395 MB |
Average GC content | 44% |
Trimmed data | |
Number of reads after trimming | 97,325,566 |
No concordantly aligned reads | 28,379,831 (29.16%) |
1-time concordantly aligned reads | 28,659,692 (29.45%) |
> 1-time concordantly aligned reads | 40,286,043 (41.39%) |
No concordantly aligns read pairs: | 28,379,831 |
1-time discordantly aligned read pairs | 9,346,861 (32.93%) |
No aligned read pairs | 19,032,970 |
Concordantly read pairs: | 38,065,940 |
Not aligned | 4,451,474 (11.69%) |
1-time aligned | 1,416,573 (3.72%) |
> 1-time aligned | 32,197,893 (84.58%) |
Overall alignment rate | 97.71% |
De novo assembly | |
Number of transcripts | 896,238 |
Number of contigs | 267,785 |