Figure 1 | Scientific Reports

Figure 1

From: Evidence the Isc iron–sulfur cluster biogenesis machinery is the source of iron for [NiFe]-cofactor biosynthesis in Escherichia coli

Figure 1

Effects of mutations in key genes of iron metabolism on hydrogenase enzyme activity. (a) In-gel hydrogenase enzyme activity determined in extracts of the indicated strains. Aliquots (25 μg of protein) of crude extracts were separated in clear-native polyacrylamide (7.5% w/v) gels. (b) Aliquots (25 μg of protein) of the same crude extracts shown in part (a) were separated under denaturing conditions in SDS-PAGE (12.5% w/v acrylamide). After transfer to a nitrocellulose membrane, the HypD polypeptide was identified (see arrow) using antiserum raised against HypD (dilution 1:4000). The asterisk denotes an unidentified cross-reacting polypeptide. (c) As in part (b) but stained with Coomassie-Brilliant-Blue. Molecular mass markers are shown in kDa on the left of panels (b,c).

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