Figure 3 | Scientific Reports

Figure 3

From: Evidence the Isc iron–sulfur cluster biogenesis machinery is the source of iron for [NiFe]-cofactor biosynthesis in Escherichia coli

Figure 3

Purified StrepII-tagged HypCD complexes isolated from strains with defects in iron metabolism. (a) Coomassie-Brilliant-Blue-stained SDS-PAGE (12.5% w/v polyacrylamide) of purified StrepII-tagged HypCD complexes (5 μg protein) isolated from the indicated strains. (b) A gel identical to that shown in part a), but challenged with antiserum containing antibodies specific for HypD (diluted 1:4000). (c) A gel similar to that shown in part (a), but with 7.5 μg of protein applied per lane and challenged with antiserum containing antibodies against HypC (diluted 1:4000). Molecular mass markers in kDa are shown on the left of each panel. Arrows identify the migration positions of HypD or HypC.

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