Figure 4 | Scientific Reports

Figure 4

From: Evidence the Isc iron–sulfur cluster biogenesis machinery is the source of iron for [NiFe]-cofactor biosynthesis in Escherichia coli

Figure 4

UV–Vis spectroscopy indicates HypD contains a [4Fe–4S] cluster. Shown are UV–Vis spectra of StrepII-tagged HypCD complexes (1 mg ml−1) isolated from the strains indicated. (a) HypCD isolated from DHP-D (∆hypD) transformed with pT-hypDCStrep (black spectrum) or from CP1244 (∆iscU) transformed with pT-hypDCStrep (gray spectrum); (b) HypCD isolated from CP1233 (∆sufA) (black spectrum) transformed with pT-hypDCStrep, or CP742 (∆iscA-∆erpA) transformed with pT-hypDCStrep (light blue spectrum).

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