Figure 5 | Scientific Reports

Figure 5

From: Evidence the Isc iron–sulfur cluster biogenesis machinery is the source of iron for [NiFe]-cofactor biosynthesis in Escherichia coli

Figure 5

Native mass spectra of HypCD complexes isolated from different E. coli mutants. (a) Native mass spectrum of native HypCD complex (upper panel) and HypCC2AD complex (lower panel) isolated from strain DHP-D (∆hypD) transformed with pT-hypDCStrep or pT-hypDC(C2A)Strep, respectively, and analysed at a collision energy of 30 V. Stoichiometry of the main complex species is StrepII-HypC:HypD of 1:1 (indicated by red circles for HypC and green circles for HypD), with minor species showing 2:1 (#) and 2:2 (*) ratios. (b) Native mass spectra of the HypCD complex isolated from strains CP1233 (∆sufA), CP1244 (∆iscU) or CP411 (∆entC-∆feoB). All strains carried plasmid pT-hypDCStrep. Signals corresponding to HypC (red overlay), HypD (green overlay) and the 1:1 complex (red-green overlay) are labeled accordingly.

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