Table 1 Strains and plasmids used in this study.

From: Evidence the Isc iron–sulfur cluster biogenesis machinery is the source of iron for [NiFe]-cofactor biosynthesis in Escherichia coli

Strain or plasmid

Relevant genotype or characteristic(s)

Reference or source

Strains

 MC4100

F− araD139 (argF-lac)U169 ptsF25 deoC1 relA1 flbB5301 rspL150

34

 XL-1 Blue

recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F’ proAB lacIqZΔM15 Tn10 (TetR)]

Stratagene

 AS10

Like MC4100, but ΔfldB (KanR)

This study

 CP411

Like MC4100, but ΔentC (CmR) feoB::Tn5

21

 CP477

Like MC4100, but ΔiscA (KanR)

4

 CP1223

Like MC4100, but ΔsufA (CmR)

4

 DHP-D

Like MC4100, but ΔhypD

8

 CP742

Like MC4100, but ∆erpA (CmR) ∆iscA

C. Pinske

 CP1244

Like MC4100, but ∆iscU

37

 MHD535

Like MC4100, but ΔcyaY

5

 PHB526

Like MC4100, but ΔiscS

5

Plasmids

 pT-hypDCStrep

pT7-7, hypD, hypCStrep, AmpR

11

 pT-hypDC(C2A)Strep

Like pT-hypDCStrep, but codon 2 in hypC changed TGC → GCG, AmpR

This study

  1. NBP National BioResources Project (NIG, Japan): E. coli.
Back to article page