Figure 5 | Scientific Reports

Figure 5

From: Engineered ice-binding protein (FfIBP) shows increased stability and resistance to thermal and chemical denaturation compared to the wildtype

Figure 5

Thermal shift assay of FfIBP and FfIBP_CC1 in various chemicals. Melting temperature (Tm) of FfIBP and FfIBP_CC1 as a function of the concentration of DMSO (a), ethylene glycol (b), methanol (c), Urea (d), propylene glycol (e), ethanol (f), and glycerol (g) was determined at emission wavelengths of 330 and 350 nm using the NanoTemper Tycho NT.6. Triplicate measurements (red or blue dots) were averaged and marked with a black line. DMSO, dimethyl sulfoxide.

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