Figure 3 | Scientific Reports

Figure 3

From: Nrf2-mediated redox balance alleviates LPS-induced vascular endothelial cell inflammation by inhibiting endothelial cell ferroptosis

Figure 3

LPS-induced ferroptosis and inflammation could be rescued by ferrostatin-1. (A) The cell viability 24 h after Fer-1 (0, 1, 4, 8, 16, and 32 μM) treatment was assayed by CCK-8; *P < 0.05, ***P < 0.001 versus 0 μM group. (B) Fer-1(1 μM) pretreated for 3 h significantly ameliorated LPS (24 h)-induced death of HUVECs; ***P < 0.001 versus Control group; #p < 0.05 versus LPS group. (C) The DCFH-DA probes were performed for LPS (3 h)-induced ROS levels (scale bar, 200 μm) after Fer-1 (1 μM) pretreated for 3 h. (D) The LPS (6 h)-induced iron ion, GSH, and MDA levels were measured after Fer-1 (1 μM) pretreated for 3 h; **P < 0.01, ***P < 0.001 versus Control group, #P < 0.05, ###P < 0.001 versus LPS group. (E) The LPS (6 h)-induced ferroptosis and inflammation-related protein expression levels were measured by western blot after Fer-1(1 μM) pretreated for 3 h; *P < 0.05, ***P < 0.001 versus Control group, #P < 0.05, ###P < 0.001 versus LPS group.

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