Figure 1
From: Multiparametric in vitro and in vivo analysis of the safety profile of self-assembling peptides
In vitro genotoxicity tests: the thymidine kinase (TK) gene and the micronucleus test assay in the TK6 human lymphoblastoid cell line. (A,B) Relative survival (RS) induced by SAPs was calculated by comparing the cloning efficiency (CE) of each SAPs with CE of the negative control determined after cultivating for 7–10 days and using the Poisson distribution method. Mitomycin C (MitC) was used as a positive control in A. (C) MF in TK-6 cells after 4 h of exposure to negative, positive controls and SAPs. (D) Photomicrographs of the cells scored in the MN assay. Arrow: a micronucleus in a mononucleated cell. (E) Percentage of micronucleated cells for each SAPs, mitomycin C (as positive control) and medium (as negative control). Data were expressed by mean ± standard deviation of the mean from three independent experiments. Statistical analyses were performed by one-way ANOVA. No significant changes regarding the control were detected.
