Figure 1

Fitness-genes after growth of the Tn-libraries generated in aminoglycoside-resistant E. coli MG1655 in the presence of STREP, GEN or NEO. (a) Venn-diagram of genes that were classified as fitness-genes based on a significant depletion of Tn5 insertions (log2FC < − 2, q-value < 0.01) was created in Excel. (b) Functional classification of aminoglycoside fitness-genes according to their associated GO terms and KEGG pathways were depicted using GraphPad Prism 9 (GraphPad Software, San Diego, USA).