Figure 2

Growth curves of the aminoglycoside-resistant ΔminCDE, ΔhflCK, ΔcpxR and ΔclsA in the presence of 700 mg/L STREP (a) and 50 mg/L GEN (b). (a) The growth of MG1655_pACYC_strAB (STREP^R) (dark blue) in LB is presented compared to the growth of the four STREP-resistant deletion mutants minCDE, hflCK, cpxR and clsA (red) in the presence of 700 mg/L STREP. (b) The growth of MG1655_aac(3)-IV(GEN^R) in LB supplemented with 50 mg/L GEN is visualized (dark blue) and compared to the growth of the four GEN-resistant deletion mutants minCDE, hflCK, cpxR and clsA (red).The mean values from four biological replicates (with four technical replicates each) and the corresponding ± standard deviation were calculated and illustrated with GraphPad Prism 9 (GraphPad Software, San Diego, USA).