Figure 4
Growth curves of ΔphoPQ, ΔwecA, Δlpp and Δpal in the presence of 1000 mg/L of NEO. The growth of MG1655_pACYC_aph(3′)-Ia(NEOR) (dark blue) compared to the growth of the four NEO-resistant deletion mutants phoPQ, wecA, lpp and pal (red) in LB supplemented with 1000 mg/L NEO is shown. Mean values from three biological replicates (with four technical replicates each) and the corresponding ± standard deviations were calculated and illustrated with GraphPad Prism 9 (GraphPad Software, San Diego, USA).
