Figure 5
Workflow of TraDIS library preparation and sequencing in an aminoglycoside-resistant E. coli MG1655. For each aminoglycoside-resistant E. coli MG1655 strain, a Tn-mutant library (input library) was created and cultured (100 µL) in LB (9.9 mL) without antibiotic (to generate the output library: control) or supplemented with a sublethal concentration of the corresponding aminoglycoside (to generate the output library: with antibiotic) at 37 °C. When an OD600 of 4 was reached, the cells were harvested, genomic DNA of the input and the two output libraries (in replicates) was extracted, prepared for sequencing and sequenced as described below. The figure was created in PowerPoint using BioRender.com.
