Table 2 List of top 30 fitness-genes in MG1655_pACYC_aac(3)-IV(GEN^R) during growth with 50 mg/L GEN.

yoeD

Pseudogene

− 6.46

0.000355

yegR

Uncharacterized protein YegR

− 6.31

0.001194

rpoH

RNA polymerase sigma factor RpoH

− 5.83

0.00593

atpC**

ATP synthase F1 complex subunit epsilon

− 4.41

4.35 × 10^–6

yoeF

Putative uncharacterized protein YoeF

− 4.11

3.7 × 10^–5

polA***

DNA polymerase I

− 3.63

1.12 × 10^–46

atpB**

ATP synthase Fo complex subunit a

− 3.50

6.76 × 10^–6

yeeS

RadC-like JAB domain-containing protein YeeS

− 3.27

5.16 × 10^–41

yajC**

Sec translocon accessory complex subunit YajC

− 3.25

7.2 × 10^–6

minE**

Z-ring positioning protein MinE

− 3.07

0.000237

cpxR**

DNA-binding transcriptional dual regulator CpxR

− 3.05

6.58 × 10^–9

pcnB***

Poly(A) polymerase I

− 2.92

2.01 × 10^–82

secF

Sec translocon accessory complex subunit SecF

− 2.88

0.000497

hflK**

Regulator of FtsH protease

− 2.88

7.51 × 10^–7

ymgK

Protein YmgK

− 2.83

0.007474

hflC**

Regulator of FtsH protease

− 2.74

1.24 × 10^–8

clsA**

Cardiolipin synthase A

− 2.72

2.31 × 10^–38

ydjJ

Putative zinc-binding dehydrogenase YdjJ

− 2.66

9.22 × 10^–5

yciC**

Putative inner membrane protein

− 2.64

2.87 × 10^–17

ispZ

Small regulatory RNA

− 2.63

7.16 × 10^–6

sspA

Stringent starvation protein A

− 2.48

0.003425

tig

Trigger factor

− 2.45

4.99 × 10^–37

atpE***

ATP synthase Fo complex subunit c

− 2.43

0.006305

ppk

Polyphosphate kinase

− 2.42

4.80 × 10^–8

yidD**

Membrane protein insertion efficiency factor

− 2.36

2 × 10^–12

fabR

DNA-binding transcriptional repressor FabR

− 2.35

5.60 × 10^–33

ppiD

Periplasmic folding chaperone

− 2.30

6.49 × 10^–11

hns

DNA-binding transcriptional dual regulator H-NS

− 2.23

4.43 × 10^–6

tyrU

One of three tyrosine tRNAs

− 2.21

2.85 × 10^–7

pstB

Phosphate ABC transporter ATP binding subunit

− 2.21

0.000225