Figure 3
From: Bone marrow stromal cell-derived hepcidin has antimicrobial and immunomodulatory activities
In vitro killing of E. coli by human or murine BMSC SNs is hepcidin-dependent. (A) Sterile filtered SNs were added to E. coli suspension and bacterial cell proliferation was followed by a microplate reader (600 nm) where one scale on the optical density (OD) axis reflects 109/mL E. coli and the OD is directly proportional to the bacterial concentration during the measurements. For human cells, polyclonal rabbit anti-hepcidin antibody or isotype control rabbit IgG was added. SN taken from BMSCs cultured from WT mice was significantly more efficient in inhibiting bacterial growth than SN of BMSCs cultured from Hamp−/− mice. (B) SN were taken off and sterile-filtered from cultured control or IL-6-stimulated human BMSCs. There was a significant decrease in E. coli proliferation when the BMSCs had been treated with 10 ng/mL IL-6 for 9 h. When hepcidin antibody was added simultaneously to the IL-6 stimulated SN, the decrease in OD was partially blocked, suggesting that hepcidin is at least partially responsible for the anti-proliferative effect of human BMSCs on E. coli. *p < 0.05; **p < 0.01; ***p < 0.001.
