Figure 2 | Scientific Reports

Figure 2

From: Calcitriol promotes M2 polarization of tumor-associated macrophages in 4T1 mouse mammary gland cancer via the induction of proinflammatory cytokines

Figure 2

Characterization of 4T1, 67NR, and E0771 cell lines used in animal studies in vitro after 72 h of treatment with 100 nM of calcitriol. (A) Real-time PCR analysis of Cyp24a1, Cyp27b1, and Vdr gene expressions. Untreated cells of each model served as a calibrator, and calculations were performed using multiple endogenous controls (Hprt, B2m, and Gapdh); N = 3. Statistical analysis: Tukey’s multiple comparisons test. (B) Densitometry analysis of target protein level; β-actin was used as the loading control. N = 3. (C) Representative blots for 4T1, 67NR, and E0771 (from left, respectively). (D) Real-time PCR analysis of the selected genes in untreated 4T1 and E0771 cell lines compared with untreated 67NR cells. Statistical analysis: Sidak’s multiple comparisons test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

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