Figure 5 | Scientific Reports

Figure 5

From: Calcitriol promotes M2 polarization of tumor-associated macrophages in 4T1 mouse mammary gland cancer via the induction of proinflammatory cytokines

Figure 5

Inflammatory cytokines secreted by TAMs and BMDMs, and tumor tissue expression of osteopontin (OPN) and epithelial cell adhesion molecule (EpCAM). (A) The concentration of IL-23 secreted by 4T1 and 67NR TAMs stimulated by 100 ng/mL LPS for 24 h. (B) IL-23 and (C). IL-6 secretion by 4T1 BMDMs. ELISA assays were conducted to evaluate the secretion of IL-23 (TAMs and BMDMs) and IL-6 for BMDMs only. Supernatants were collected after 24 h of 100 ng/mL LPS stimulation. (D) Western blot evaluation of OPN and EpCAM levels in 4T1 tumor tissue homogenates. (E) Representative blots of OPN and EpCAM tumor tissue analysis: 1–1000 IU, 2–1000 IU + cal, 3–5000 IU, 4–100 IU, and 5–100 IU + cal. The number of repeats is presented on the graph. Statistical analysis: Sidak’s multiple comparison test; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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