Figure 7
Expression of selected proteins in BMDMs cultured with 4T1 culture supernatants ex vivo. BMDMs were derived from healthy mice fed diets of varying contents of VD3 and treated with calcitriol. BMDMs were differentiated in the presence of 50 ng/mL M-CSF and 40% v/v CM-conditioned medium. CM was derived from 100 nM calcitriol-stimulated (4T1cal CM) or not (4T1 CM) 4T1 cells. Before lysate collection, BMDMs were stimulated for 24 h with 100 ng/nL LPS. (A–E) Western blot analysis (N = 3) of (A) IRF4, (B) MMP3, (C) EpCAM, and (D) TGF-β. (E) Representative blots; 1–control, 2–4T1 CM, and 3–4T1cal CM. (F) Concentration of IL-6 in BMDMs culture media supernatants. N = 3. Statistic: Sidak's multiple comparisons test; *P < 0.05, **P < 0.01, ***P < 0.001.
