Table 1 TaqMan™ gene expression assays are utilized for RT-PCR.

From: Calcitriol promotes M2 polarization of tumor-associated macrophages in 4T1 mouse mammary gland cancer via the induction of proinflammatory cytokines

Gene

Assay number

Gapdh

Mm99999915_g1

Hprt

Mm00446968_m1

B2m

Mm00437762_m1

Cyp27b1

Mm01165918_g1

Cyp24a1

Mm00487244_m1

Vdr

Mm00437297_m1

Il6

Mm00446190_m1

Il23

Mm00518984_m1

Irf4

Mm00516431_m1

Fn1

Mm01256744_m1

Mrc1

Mm01329362_m1

Tgfb1

Mm01178820_m1

Tnfa

Mm00443258_m1

Arg1

Mm00475988_m1

Infg

Mm01168134_m1

Spp1

Mm00436767_m1

  1. Endogenous controls: Glyceraldehyde-3-phosphate dehydrogenase (Gapdh); hypoxanthine phosphoribosyltransferase 1 (Hprt); and beta-2-microglobulin (B2m). Target genes: cytochrome P450 family 27 subfamily B member 1 (Cyp27b1); cytochrome P450, family 24, subfamily a, polypeptide 1 (Cyp24a1); vitamin D receptor (Vdr); interleukin 6 (Il6), interleukin 23 (Il23), interferon regulatory factor 4 (Irf4); fibronectin 1 (Fn1); mannose receptor C-type 1 (Mrc1); transforming growth factor-beta 1 (Tgfb1); tumor necrosis factor alpha (Tnfa); arginase 1 (Arg1); interferon gamma (Infg); and osteopontin (Spp1).
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