Table 1 Analytical parameters for spectrofluorometric assessment of BHT and BHA.

Wavelength (nm)

293

317

317

317

Linear range

3–18 µg/mL

0.1–7 µg/mL

2–30 mg/kg

0.25–10 mg/L

Determination coefficient

0.9995

0.9991

0.9961

0.9986

Intercept

10,979

6162.8

 − 283.19

1538

Slope

4585.1

87,302

2028.2

24,545

LOD (mg/mL)

0.9 µg/mL

0.02 µg/mL

0.7 mg/kg

0.1 mg/L

LOQ (mg/mL)

3 µg/mL

0.05 µg/mL

2 mg/kg

0.25 mg/L

Repeatability (% RSD)

1.01^a

1.92^a

7.6^c

6.24^c

Intermediate precision (% RSD)

1.5^b

1.95^b

7.06^d

6.62^d

Concentration of pre-existing BHA in butter oil (mg/kg ± mg/kg)

–

8.64 mg/kg ± 0.4 mg/kg^e

–

–