Figure 2

IL-1β is required for contractions of collagen lattices of primary human myometrial cells co-cultured with macrophages. (a) Schematic representation of the LPS-mediated IL-1β effects on myometrial cells contraction cultured with macrophages. (b) IL-1β secretion in co-culture cell medium. Macrophages and myometrial cells were cultured for 24 h in presence or absence of LPS (100 ng/ml). Supernatants were collected and IL-1β was evaluated with ELISA assay. Concentrations were standardized with protein cell content and presented as means ± SEM, n = 6, **P < 0.01. (c) Representative photographs of collagen lattices of myometrial cells co-cultured with macrophages stimulated with LPS (100 ng/ml), in presence or absence of Anakinra (10 µg/ml) or αIL-1β (1 µg/ml) at 96 h. (d) Mean percentage contraction of collagen lattices after 96 h of stimulation with LPS or LPS + Anakinra (10 µg/ml) or LPS + αIL-1β (1 µg/ml) compared to the basal surface area, mean ± SEM, n = 5, *P < 0.05, **P < 0.01 and ****P < 0.0001. (e) Schematic representation of the IL-1β effects on myometrial cells contraction. f) Photographs of collagen lattices of myometrial cells alone in presence of LPS (100 ng/ml) or IL-1β (1 ng/ml). (g) Mean percentage contraction of collagen lattices ± SEM after 96 h of LPS or IL-1β treatment, compared to the basal surface area.