Figure 4

Neutralizing IL-1β effect prevents the differentiation of primary human myometrial smooth muscle cells. (a) Schematic representation of the LPS effects on differentiation of myometrial cells in a co-culture model. (b) Representative images of myometrial cells and macrophages co-cultures treated with LPS (100 ng/ml) in presence or absence of Anakinra (1 µg/ml) or αIL-1β (100 ng/ml) and stained with phalloidin (green), vinculin (purple) and DAPI (blue) for nuclear localization. Images, taken with an Axiozoom epifluorescence microscope (×400), are representative of 5 random pictures of four independent experiments. (c) Mean fluorescence intensity of phalloidin or vinculin ± SEM, n = 20, **P < 0.01, ***P < 0.001, ****P < 0.0001. (d) Fluorescence images of LY (green) and RD (red) transfer to adjacent cells after scrap loading. Cells were treated for 24 h with LPS ± Anakinra or αIL-1β. Pictures were taken with an inverted microscope at ×100 magnification in two random chosen fields and are representative of three experiments. (e) Mean surface of LY staining ± SEM, n = 6, ****P < 0.0001.