Figure 6

Inhibition of NLRP3 inflammasome prevents LPS-induced contractions of collagen lattices of primary human myometrial cells co-cultured with macrophages. (a) Schematic representation of the effects of NLRP3 inflammasome inhibition on differentiation of myometrial cells in a co-culture model. (b) Macrophages and myometrial cells were co-cultured for 24 h in presence or absence of LPS (100 ng/ml) ± MCC950 (0.1 µM). Supernatants were collected and IL-1β concentrations were evaluated with ELISA assay and standardized with protein cell content ± SEM, n = 6, *P < 0.05 and **P < 0.01. (c) Representative photographs of collagen lattices of myometrial cells co-cultured with macrophages stimulated with LPS (100 ng/ml) ± MCC950 for 96 h. (d) Mean percentage contraction of collagen lattices after 96 h of stimulation with LPS in presence or absence of MCC950 compared to the basal surface area, mean ± SEM, n = 5, *P < 0.05.